Use of microplates for monolayer tissue cultures.

Study of Expression Level of Cartilage Genes in Rat Articular Chondrocyte Monolayer and 3D Cultures using Real Time PCR

Purpose: to compare the expression level of certain genes related to cartilage and non-cartilage tissues at monolayer and alginate cultures derived from rat articular cartilage. Materials and Methods: Articular cartilage was harvested from knee joints of 10 male rats and was digested using enzymatic solution consisting of 0.2% collagenase I and 0.1% pronase. Released chondrocyte were then plate...

Human 3-D tissue models in radiation biology: current status and future perspectives

In this review, we discuss the use of a variety of 3-D models (particularly 3-D skin, lung, breast and endothelial) in radiobiological research and highlight the differences in responses compared to 2-D culturing conditions (monolayers). We review the characteristics of existing 3-D models and aim to point out the substantial advantages 3-D cultures provide for modern radiobiology. In particula...

Propagation of goat pox virus on monolayer cell cultures

Reduced DNA damage in tumor spheroids compared to monolayer cultures exposed to ionizing radiation

Background: Several cell lines when cultured under proper condition can form three dimensional structures called multicellular tumor spheroids. Tumor spheroids are valuable in vitro models for studying physical and biological behavior of real tumors. A number of previous studies using a variety of techniques have shown no relationship between radiosensitivity and DNA strand breaks in monolayer ...

Establishment of a Primary Cell Culture of Human Fibroblast in Iran

Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic and varicose vein leg ulcers. Therefore, we aimed at developing a fibroblast bank to acco...